poly a extraction kit microfasttrack Search Results


microfasttrack mrna isolation kit  (Thermo Fisher)
90
Thermo Fisher microfasttrack mrna isolation kit
Microfasttrack Mrna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfasttrack mrna isolation kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
microfasttrack mrna isolation kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
microfasttrack 2.0 mrna isolation kit  (Thermo Fisher)
90
Thermo Fisher microfasttrack 2.0 mrna isolation kit
Microfasttrack 2.0 Mrna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfasttrack 2.0 mrna isolation kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
microfasttrack 2.0 mrna isolation kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
microfasttrack 2 0 mrna isolation kit  (Thermo Fisher)
99
Thermo Fisher microfasttrack 2 0 mrna isolation kit
Microfasttrack 2 0 Mrna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfasttrack 2 0 mrna isolation kit/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
microfasttrack 2 0 mrna isolation kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
messenger rna  (Thermo Fisher)
99
Thermo Fisher messenger rna
Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and <t>RNA</t> hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. <t>Messenger</t> <t>RNA</t> (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.
Messenger Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/messenger rna/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
messenger rna - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
microfastrack kit  (Thermo Fisher)
90
Thermo Fisher microfastrack kit
Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and <t>RNA</t> hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. <t>Messenger</t> <t>RNA</t> (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.
Microfastrack Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfastrack kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
microfastrack kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
micro fasttrack microfasttrack kit  (Thermo Fisher)
86
Thermo Fisher micro fasttrack microfasttrack kit
Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and <t>RNA</t> hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. <t>Messenger</t> <t>RNA</t> (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.
Micro Fasttrack Microfasttrack Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/micro fasttrack microfasttrack kit/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
micro fasttrack microfasttrack kit - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier
microfasttrack kit  (Thermo Fisher)
90
Thermo Fisher microfasttrack kit
Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and <t>RNA</t> hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. <t>Messenger</t> <t>RNA</t> (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.
Microfasttrack Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfasttrack kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
microfasttrack kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
microfasttrak  (Thermo Fisher)
90
Thermo Fisher microfasttrak
Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and <t>RNA</t> hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. <t>Messenger</t> <t>RNA</t> (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.
Microfasttrak, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfasttrak/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
microfasttrak - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
microfasttracktm 2.0 kit  (Thermo Fisher)
90
Thermo Fisher microfasttracktm 2.0 kit
Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and <t>RNA</t> hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. <t>Messenger</t> <t>RNA</t> (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.
Microfasttracktm 2.0 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfasttracktm 2.0 kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
microfasttracktm 2.0 kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and RNA hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. Messenger RNA (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.

Journal:

Article Title: Impaired neuromuscular transmission and skeletal muscle fiber necrosis in mice lacking Na/Ca exchanger 3

doi: 10.1172/JCI200418688

Figure Lengend Snippet: Targeted disruption of the Ncx3 gene. (a) Structure of the WT and the targeted alleles. The second exon (shaded boxes), the neomycin resistance cassette (black box), the probes used in DNA and RNA hybridization analysis (black bars underneath the second exon), as well as the DNA fragments generated after digestion with KnpI + SpeI or KpnI alone are represented. K, KpnI; H, HindIII; S, SpeI; E, EcoRI; B, BamHI. (b and c) DNA hybridization analysis of KpnI + SpeI– (b) and KpnI- (c) digested genomic DNA isolated from ES clones using the depicted probe. (d) RNA hybridization analysis. Messenger RNA (0.5 μg/lane) was hybridized with Ncx3 or GAPDH RNA probes. (e) RT-PCR analysis. Ncx3 amplicon (∼600 bp) and housekeeping gene HPRT amplicon (∼250 bp) were simultaneously amplified from gastrocnemius muscle messenger RNA by one-step RT-PCR. (f, g, and h) Western blot analysis. (f) Membrane fractions (50 μg/lane) from gastrocnemius muscle were analyzed with anti-NCX3–specific antibody. (g) Immunodetection of membrane fractions from Ncx3+/+ (lanes 1–3) and Ncx3–/– (lanes 4–6) gastrocnemius muscle analyzed with anti-NCX1– and anti-PMCA1–specific antibodies. Amount of protein loaded: lanes 1 and 4, 50 μg; lanes 2 and 5, 15 μg; lanes 3 and 6, 5 μg. (h) Membrane fractions (20 μg/lane) from FDB muscle were analyzed with anti-NCX1–specific antibody. Protein loading in (f–h) was determined by using a mouse anti-desmin monoclonal antibody.

Article Snippet: Messenger RNA (0.5 μg, MicrofastTrack 2.0 kit, Invitrogen, Carlsbad, California, USA) was extracted from gastrocnemius muscles.

Techniques: Hybridization, Generated, DNA Hybridization, Isolation, Clone Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Western Blot, Immunodetection